fabp3  (Sanying Ltd)

Journal: Journal of Sport and Health Science

Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice

doi: 10.1016/j.jshs.2025.101095

Figure Lengend Snippet: Impact of high fat feeding and voluntary exercise on left ventricle morphology in male mice. (A) Representative images of Picrosirius red staining for measurement of interstitial and perivascular fibrosis with 20× magnification. (B) Representative images for Hematoxylin and Eosin for measurement of cardiomyocyte width and area with 40× magnification. (C) Representative images of Oil red O staining for measurement of interstitial lipid deposition with 40× magnification. Quantitative analysis of percent area of (D) interstitial and (E) perivascular fibrosis expressed as fold change from sedentary chow group. Quantitative analysis of (F) cardiomyocyte width and (G) cardiomyocyte area. (H) Quantitative analysis of percent area of lipid deposition expressed as fold change from sedentary chow group left ventricle mRNA expression of fatty acid transporters (I) FABP3, (J) CD36, and hypertrophy markers (K) β-MHC and (L) ANP in male VET or sedentary mice fed an HFD or chow diet. Values were calculated relative to 18S housekeeper. Analysis was performed using two-way analysis of variance with Tukey’s post hoc test for multiple comparisons. Data are expressed as mean ± standard error of the mean. (D–H) n : 8–12 per group. (I–L) n : 8–9 per group. * p < 0.05, **** p < 0.0001. ANP = atrial natriuretic peptide; β-MHC = beta myosin heavy chain; CD36 = platelet glycoprotein 4; FABP3 = fatty acid-binding protein 3; HFD = high fat diet; Myh7 = moysin heavy chain 7; VET = voluntary exercise training.

Article Snippet: The following TaqMan assay gene transcripts were used: fatty acid-binding protein 3 (FABP3, Mm02342495_m1), platelet glycoprotein 4 (CD36, Mm00432403_m1), beta myosin heavy chain (β-MHC, Mm00600555_m1), atrial natriuretic peptide (ANP, Mm01255747_g1), OPA1 (Mm01349707_g1), DRP1 (Mm01342903_m1), robosomal18S (18S, Mm03928990_g1).

Techniques: Staining, Expressing, Binding Assay

Journal: Drug Design, Development and Therapy

Article Title: Atorvastatin Attenuates Vancomycin-Induced Nephrotoxicity via PPARα-Associated Regulation of SLC Transporters

doi: 10.2147/DDDT.S571916

Figure Lengend Snippet: Validation of DEGs associated with the PPAR signaling pathway by qRT-PCR. ( A-H ) Validation of DEGs related to the PPAR signaling pathway, including CYP4A10 , ACOX2 , SLC27A2 , FABP3 , ACAA1B , APOC3 , CYP4A31 , and GK (panels A-H, respectively). The data are presented as mean ± SD, n = 6. Statistical analyses were performed using unpaired Student’s t -test for comparisons between the VCM model group and the VCM + ATO (10 mg/kg) group. In transcriptome sequencing, compared with the VCM model group, ** P < 0.01, *** P < 0.001; in qRT-PCR, compared with the VCM model group, # P < 0.05, ## P < 0.01, ### P < 0.001.

Article Snippet: All antibodies employed in the present study were as follows: Bcl-2 (AF6139, 1:1000 dilution, Affinity Biosciences, USA), Bax (AF0120, 1:1000 dilution, Affinity Biosciences, USA), FABP3 (10,676-1-AP, 1:2000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China), ACOX2 (17,571-1-AP, 1:1000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China), FATP2 (14,048-1-AP, 1:2000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China), OAT1 (26,574-1-AP, 1:1000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China), OCT2 (18,996-1-AP, 1:1000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China), OAT3 (16,844-1-AP, 1:1000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China), MATE1 (20,898-1-AP, 1:1000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China), β-actin (20536-1-AP, 1:5000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China), and horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (H + L) secondary antibody (SA00001-2, 1:5000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China).

Techniques: Biomarker Discovery, Quantitative RT-PCR, Sequencing

Journal: Drug Design, Development and Therapy

Article Title: Atorvastatin Attenuates Vancomycin-Induced Nephrotoxicity via PPARα-Associated Regulation of SLC Transporters

doi: 10.2147/DDDT.S571916

Figure Lengend Snippet: Validation of DEGs related to the PPAR signaling pathway and SLC-mediated transmembrane transport by Western blot.Validation of DEGs related to the PPAR signaling pathway by Western blot. ( A ) Western blot analysis of FABP3, ACOX2, and FATP2 protein expression in renal tissues; ( B ) Western blot analysis of OAT1, OCT2, OAT3, and MATE1 protein expression in renal tissues.

Article Snippet: All antibodies employed in the present study were as follows: Bcl-2 (AF6139, 1:1000 dilution, Affinity Biosciences, USA), Bax (AF0120, 1:1000 dilution, Affinity Biosciences, USA), FABP3 (10,676-1-AP, 1:2000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China), ACOX2 (17,571-1-AP, 1:1000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China), FATP2 (14,048-1-AP, 1:2000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China), OAT1 (26,574-1-AP, 1:1000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China), OCT2 (18,996-1-AP, 1:1000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China), OAT3 (16,844-1-AP, 1:1000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China), MATE1 (20,898-1-AP, 1:1000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China), β-actin (20536-1-AP, 1:5000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China), and horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (H + L) secondary antibody (SA00001-2, 1:5000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China).

Techniques: Biomarker Discovery, Western Blot, Expressing

Journal: Drug Design, Development and Therapy

Article Title: Atorvastatin Attenuates Vancomycin-Induced Nephrotoxicity via PPARα-Associated Regulation of SLC Transporters

doi: 10.2147/DDDT.S571916

Figure Lengend Snippet: Mechanistic study of the protective effects of ATO against VIN. ( A ) GW6471 (10 μM) reverses the cytoprotective effect of ATO against VCM-induced injury; ( B ) qRT-PCR analysis of PPARα pathway downstream target genes; ( C ) qRT-PCR analysis of SLC transporter genes. The data are presented as mean ± SD, n = 3. Statistical analyses were performed using Tukey’s post hoc test for all pairwise comparisons. Compared with the CONTROL group, *** P < 0.001; compared with the VCM model group, # P < 0.05, ## P < 0.01, ### P < 0.001; compared with the VCM + ATO (10 μM) group, & P < 0.05, && P < 0.01, &&& P < 0.001. ( A ) CONTROL vs. VCM, P < 0.0001; VCM vs. VCM + ATO (10 μM), P = 0.0002; VCM + ATO (10 μM) vs. VCM + ATO (10 μM) + GW6471, P = 0.0032. (B-FABP3) VCM vs. VCM + ATO (10 μM), P = 0.0028; VCM + ATO (10 μM) vs. VCM + ATO (10 μM) + GW6471, P = 0.0002. (B-AOX2) VCM vs. VCM + ATO (10 μM), P = 0.0055; VCM + ATO (10 μM) vs. VCM + ATO (10 μM) + GW6471, P = 0.0153. (B-SLC27A2) VCM vs. VCM + ATO (10 μM), P = 0.0001; VCM + ATO (10 μM) vs. VCM + ATO (10 μM) + GW6471, P < 0.0001. (C-SLC22A6) VCM vs. VCM + ATO (10 μM), P = 0.0115; VCM + ATO (10 μM) vs. VCM + ATO (10 μM) + GW6471, P = 0.0084. (C-SLC22A2) VCM vs. VCM + ATO (10 μM), P < 0.0001; VCM + ATO (10 μM) vs. VCM + ATO (10 μM) + GW6471, P = 0.0001. (C-SLC22A8) VCM vs. VCM + ATO (10 μM), P = 0.0347; VCM + ATO (10 μM) vs. VCM + ATO (10 μM) + GW6471, P = 0.0148. (C-SLC47A1) VCM vs. VCM + ATO (10 μM), P = 0.0034; VCM + ATO (10 μM) vs. VCM + ATO (10 μM) + GW6471, P = 0.0009.

Article Snippet: All antibodies employed in the present study were as follows: Bcl-2 (AF6139, 1:1000 dilution, Affinity Biosciences, USA), Bax (AF0120, 1:1000 dilution, Affinity Biosciences, USA), FABP3 (10,676-1-AP, 1:2000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China), ACOX2 (17,571-1-AP, 1:1000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China), FATP2 (14,048-1-AP, 1:2000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China), OAT1 (26,574-1-AP, 1:1000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China), OCT2 (18,996-1-AP, 1:1000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China), OAT3 (16,844-1-AP, 1:1000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China), MATE1 (20,898-1-AP, 1:1000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China), β-actin (20536-1-AP, 1:5000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China), and horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (H + L) secondary antibody (SA00001-2, 1:5000 dilution, SanYing Biotechnology Co., Ltd., Wuhan, China).

Techniques: Quantitative RT-PCR, Control

Journal: Cell Discovery

Article Title: Luminal hormone-responsive cells tune the regenerative remodeling of mammary glands in large mammals

doi: 10.1038/s41421-025-00848-3

Figure Lengend Snippet: a UMAP plot displaying 14 identified cell types within the goat mammary glands. Cells are annotated and colored by type. Subsets of cell types including luminal, basal, fibroblast, immune, endothelial cell are labeled. b The percentages of −4W (red) and +1 W (blue) cells in each cell type are shown in a UMAP plot. c UMAP plots showing the expression of selected marker genes in four luminal subtypes. d Pseudotemporal trajectory analysis of scRNA-seq data of luminal secretory cells is shown in a UMAP plot. e Changes in the proportion of LumSecP and LumSec cells in luminal cell types were identified by scRNA-seq data at −4W and +1 W. n = 3 goats per group. f Representative images of tissue immunofluorescence staining for ALDH1A3 (red), KRT18 (green), and DAPI at −4W and +1 W. Scale bars, 50 μm. g Bar plots exhibiting the percentage of ALDH1A3-positive cells in luminal cells (labeled by KRT18) in f . n = 15 sections per group. h Representative images of tissue immunofluorescence staining for FABP3 (red), KRT18 (green), and DAPI at −4W and +1 W. Scale bars, 50 μm. i Bar plots exhibiting the percentage of FABP3-positive cells in luminal cells (labeled by KRT18) in h . n = 20 sections per group. The data are presented as the mean ± SEM. The P values of two-sided Student’s t -tests are shown in e , g , i .

Article Snippet: For immunofluorescence staining, goat mammary tissues were stained with primary antibodies against ALDH1A3 (#25167-1-AP, Proteintech), FABP3 (#10676-1-AP, Proteintech), KRT17 (#17516-1-AP, Proteintech), KRT14 (#SC65-06, HUABIO), KRT18 (conjugated with iFluorTM 488, #SZ80-07, HUABIO), KRT8 (#10384-1-AP, ProteinTech), PR (#25871-1-AP, ProteinTech), ER (#21244-1-AP, ProteinTech), and PCNA (#ab29, Abcam).

Techniques: Labeling, Expressing, Marker, Immunofluorescence, Staining

Journal: Cell Discovery

Article Title: Luminal hormone-responsive cells tune the regenerative remodeling of mammary glands in large mammals

doi: 10.1038/s41421-025-00848-3

Figure Lengend Snippet: a Relative proportions of LumHR cells in total luminal cells identified by scRNA-seq data at −4W and +1 W. n = 3 goats per group. b , c Bar plots exhibiting the percentage of PGR - and ESR1 -positive cells within luminal cells in scRNA-seq data. n = 3 goats per group. d Representative images of immunofluorescence staining for PR (red), KRT18 (green), and DAPI (blue). Scale bars, 50 μm. e Bar plots exhibiting the percentage of PR-positive cells in luminal cells (labeled by KRT18) in ( d ). n = 5 goats per group. f Representative images of immunofluorescence staining for ER (red), KRT18 (green), and DAPI (blue). Scale bars, 50 μm. g Bar plots exhibiting the percentage of ER-positive cells in luminal cells (labeled by KRT18) in ( f ). n = 5 goats. h Violin plot showing the specific expression of PRLR in LumHR cells by scRNA-seq. i Heatmap displaying the transcriptional level of indicated genes related to milk protein and luminal differentiation in the goat mammary organoids ( n = 3 biological replicates) treated with or without prolactin and in the mammary tissue at −4W (non-lactation) and +1 W (lactation). n = 3 goats for tissues. j Proportions of luminal cell types in goat mammary organoids incubated with or without prolactin predicted by CIBERSORTx deconvolution. n = 3 biological replicates. k Representative images of immunofluorescence staining for ALDH1A3 (red), KRT18 (green), and DAPI in mammary organoids incubated with or without prolactin. Scale bars, 10 μm. l Bar plots exhibiting the percentage of ALDH1A3-positive cells in luminal cells (labeled by KRT18) in ( k ). n = 14 domes in the control group and n = 10 domes in the prolactin treated group. m Representative images of immunofluorescence staining for FABP3 (red), KRT18 (green) and DAPI in mammary organoids incubated with or without prolactin. Scale bars, 10 μm. n Bar plots exhibiting the percentage of FABP3-positive cells in luminal cells (labeled by KRT18) in ( m ). n = 6 domes in the control group and n = 7 domes in the prolactin-treated group. The data are presented as the mean ± SEM. The P values of two-sided Student’s t -tests are shown in ( a – c , e , g , j , l , n ).

Article Snippet: For immunofluorescence staining, goat mammary tissues were stained with primary antibodies against ALDH1A3 (#25167-1-AP, Proteintech), FABP3 (#10676-1-AP, Proteintech), KRT17 (#17516-1-AP, Proteintech), KRT14 (#SC65-06, HUABIO), KRT18 (conjugated with iFluorTM 488, #SZ80-07, HUABIO), KRT8 (#10384-1-AP, ProteinTech), PR (#25871-1-AP, ProteinTech), ER (#21244-1-AP, ProteinTech), and PCNA (#ab29, Abcam).

Techniques: Immunofluorescence, Staining, Labeling, Expressing, Incubation, Control

Journal: Cell Discovery

Article Title: Luminal hormone-responsive cells tune the regenerative remodeling of mammary glands in large mammals

doi: 10.1038/s41421-025-00848-3

Figure Lengend Snippet: a Schematic illustration of targeted ablation of LumHR cells using the prlr -promoter to drive expression of DTA. b Experimental setup used in AAV intraductally injected mammary gland of ROSA-DTA +/ − mice under RR. c Whole-mount staining with carmine alum of mammary glands from ROSA-DTA +/ − mice (lactation day 2) intraductally injected with AAV-pPrlr-Cre or AAV-Control . Scale bars, 4 mm (top) and 500 μm (bottom). d , e Immunohistochemical staining and quantification of ER-positive luminal cells in mammary glands intraductally injected with AAV-pPrlr-Cre or AAV-Control. n = 3 mice per group. Scale bars, 50 μm. f , g Immunohistochemical staining and quantification of PR-positive luminal cells in mammary glands intraductally injected with AAV-pPrlr-Cre or AAV-Control . n = 3 mice per group. Scale bars, 50 μm. h , i Immunohistochemical staining and quantification of β-casein-positive alveoli number per mm 2 in mammary glands intraductally injected with AAV-pPrlr-Cre or AAV-Control . n = 3 mice per group. Scale bars, 50 μm. j Representative images of immunofluorescence staining for FABP3 (red), KRT18 (green), and DAPI in mammary glands intraductally injected with AAV-pPrlr-Cre or AAV-Control . Scale bars, 50 μm. k Bar plots exhibiting the percentage of FABP3-positive cells in luminal cells (labeled by KRT18) in j . n = 3 mice per group. l Representative images of immunofluorescence staining for ALDH1A3 (red), KRT18 (green), and DAPI in mammary glands intraductally injected with AAV-pPrlr-Cre or AAV-Contro l. Scale bars, 50 μm. m Bar plots exhibiting the percentage of FABP3-positive cells in luminal cells (labeled by KRT18) in ( l ). n = 3 mice per group. The data are presented as the mean ± SEM. The P values of two-sided Student’s t -tests are shown in d , g , i , l , m .

Article Snippet: For immunofluorescence staining, goat mammary tissues were stained with primary antibodies against ALDH1A3 (#25167-1-AP, Proteintech), FABP3 (#10676-1-AP, Proteintech), KRT17 (#17516-1-AP, Proteintech), KRT14 (#SC65-06, HUABIO), KRT18 (conjugated with iFluorTM 488, #SZ80-07, HUABIO), KRT8 (#10384-1-AP, ProteinTech), PR (#25871-1-AP, ProteinTech), ER (#21244-1-AP, ProteinTech), and PCNA (#ab29, Abcam).

Techniques: Expressing, Injection, Staining, Control, Immunohistochemical staining, Immunofluorescence, Labeling